ÓÄÊ 613.5 614.3 614.4
DOI 10.33861/2071-8020-2025-6-42-44
Original Empirical Research
Kovalevskaya M. A., Shakhova V. N., Svetlakova E. V., Ozheredova N. A., Dmitriev A. F.
Abstract. The problem of hygiene and cleanliness is of paramount importance in the modern world. In many industries, hygiene and biocontamination control are essential for the production of safe products with stable properties. International trade in products, the quality of which depends on compliance with hygiene and cleanliness requirements, is growing. The aim of the study to conduct microbiological control of swabs from process clothing and swabs from hands of personnel admitted to work in clean rooms. Methods. In clean rooms, a swab method was used from the surface of a work suit in four places: one swab from the hands, shoulders and helmet to determine the microbial contamination of the gloves and clothing of the working personnel. Fingerprints were made on an agar nutrient medium - one print of each finger was left, or a quick movement from top to bottom was made. Then the cups were thermostatted and a colony count was made – for microbiological control of the hands of the personnel. Microbial contamination of clothing was determined using contact plates, which were applied to certain areas of the worker’s body, then thermostatted and counted. The level of colonyforming units on the gloves was less than 1. This indicates strict compliance with the rules of hygiene and hand disinfection by specialists working in clean rooms, including machine operators and microbiologists. The results obtained fully comply with the requirements of internal regulations and GMP standards, which confirms the excellent level of sanitary safety and the effectiveness of the adopted sanitary measures.
Keywords: personnel, swabs, microbial contamination, clean rooms, technological clothing, control, GMP.
Author affiliation:
Kovalevskaya Maria A., student of the Institute of Veterinary Medicine and Biotechnology of the Stavropol State Agrarian University; 12, Zootechnicheskiy lane, Stavropol, 300017; e-mail: kov.manya@yandex.ru.
Svetlakova Elena V., Ph. D. in Biology, Associate Professor of the Department of Epizootology and Microbiology of the Stavropol State Agrarian University; 12, Zootechnicheskiy lane, Stavropol, 300017; phone: 8-903-416-83-55; e-mail: alenka61219@mail.ru.
Ozheredova Nadezhda A., D. Sc. in Veterinary Medicine, professor, Head of the department of Epizootology and Microbiology of the Stavropol State Agrarian University; 12, Zootechnicheskiy lane, Stavropol, 300017; e-mail: ogeredova-sgau@mail.ru.
Dmitriev Anatoly F., D. Sc. in Biology, Professor, Professor of the Department of Epizootology and Microbiology of the Stavropol State Agrarian University; 12, Zootechnicheskiy lane, Stavropol, 300017.
Responsible for correspondence with the editorial board: Shakhova Valeria N., D.Sc. in Biology, Associate Professor, Associate Professor of the Department of Therapy and Pharmacology of the Stavropol State Agrarian University; 12, Zootechnicheskiy lane, Stavropol, 300017; e-mail: lerik_perev@mail.ru.
Authors’ Contribution: the manuscript was written with the input of all authors. All authors approved the final version of the manuscript.
Conflict of Interest Statement: the authors declare no conflict of interest.
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