ÓÄÊ 619:616.921.5:636.5:639.127
DOI 10.33861/2071-8020-2025-6-15-18
Original Empirical Research
Poryvaeva A. P., Tomskikh O. G., Krasnoperov A. S., Oparina O. Yu., Nurmieva V. R.
Abstract. The problem of the globalization of the spread of highly virulent avian influenza viruses in the world and the emergence of a pandemic variant of the A(H5N1) virus, despite the efforts of the global scientific community, has not lost its urgency and relevance for more than a quarter of a century. This article presents an analysis of retrospective and operational data from diagnostic studies conducted as part of state epizootic monitoring of the circulation of influenza A pathogens, the natural reservoir of which are migratory waterfowl and aquatic birds. It is shown that agricultural facilities in the Sverdlovsk region are at risk for the spread of influenza A viruses, as most of them are located either at the intersection of major migration routes of waterfowl and aquatic birds or in their primary nesting areas. This increases the risk of transmission of influenza A pathogens from wild birds to domestic birds and livestock. Based on our own research, we found that antibodies to avian influenza A virus strains were present in 0.8% of blood serum samples from cattle from livestock farms located in the nesting areas of migratory waterfowl in the Sverdlovsk region. A negative result was obtained in blood serum samples tested using enzyme-linked immunosorbent assay (ELISA), and no H5/H7/H9 influenza A virus genome was detected in nasal swab samples from these animals using polymerase chain reaction. No H5/ H7/H9 influenza A virus circulation was detected in biological samples from synanthropic birds and wild ruminants. Therefore, analysis of the study results confirmed the free status of the livestock farms in the Sverdlovsk region surveyed for avian influenza.
Keywords: antibody dynamics, avian influenza virus, cattle, wild ungulates, migratory waterfowl, synanthropic birds, Sverdlovsk region, hemagglutination inhibition reactions, enzyme-linked immunosorbent assay, molecular genetic studies.
Author affiliation:
Poryvaeva Antonina P., D.Sc. in Biology, Leading Scientific Researcher of the Department of Monitoring and Forecasting of Infectious Diseases of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112 a, Belinskogo st., Yekaterinburg, 620142; phone: 8-922-1122095; e-mail: app1709@inbox.ru.
Tomskikh Oksana G., Ph.D. in Veterinary Medicine, Senior Scientific Researcher of the Department of Monitoring and Forecasting of Infectious Diseases of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112 a, Belinskogo st., Yekaterinburg, 620142; phone: 8-9068087191; e-mail: tomskiy1982@mail.ru.
Oparina Olga Yu., Ph.D. in Veterinary Medicine, Senior Scientific Researcher of the Department of Ecology and Non-Infectious Pathology of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112 a, Belinskogo st., Yekaterinburg, 620142; phone: 8-912-6011634; e-mail: olia91oparina@yandex.ru.
Nurmieva Venera R., Research Engineer of the Department of Monitoring and Forecasting of Infectious Diseases of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112 a, Belinskogo st., Yekaterinburg, 620142; phone: 8-951-1269589; e-mail: verba.6255@mail.ru.
Responsible for correspondence with the editorial board: Krasnoperov Aleksander S., Ph.D. in Veterinary Medicine, Senior Scientific Researcher of the Department of Ecology and Non-Infectious Pathology of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112 a, Belinskogo st., Yekaterinburg, 620142; phone: 8-903-0833132; e-mail: marafon.86@list.ru.
Authors’ Contribution: the manuscript was written with the input of all authors. All authors approved the final version of the manuscript.
Conflict of Interest Statement: the authors declare no conflict of interest.
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