New Lactobacillus Acidophilus strains as promising probiotics for poultry farming

УДК 579.64
DOI 10.33861/2071-8020-2022-6-16-21

Valiullin L.R., Mukhammadiev Rish.S., Mukhammadiev Rin.S. Timerbaeva R.R., Karimullina I.G., Yarullin A.I.

Summary. Authors conducted a study of the probiotic properties of new strains of L. acidophilus in vitro to determine the possibility of their use in poultry farming. Six strains of L. acidophilus were studied, the antagonistic potential of which was determined by the plate method. The strains L. acidophilus IV8 and L. acidophilus IV72 had relatively high antimicrobial activity against pathogens of intestinal infections in young poultry. Incubation of their bacterial cells (2.60x109 and 5.14x109 CFU/mL, respectively) in liquid MRS medium containing 1 and 5% broiler bile for 6 h showed their resistance. In the medium with 1% bile, the survival rate of strains IV8 and IV72 was 41.9 and 26.3%, respectively, in the presence of 5% bile, 18.0 and 8.4% relative to the control. Determination of the resistance of lactobacilli in media with approximate pH values to the conditions of various parts of the birds’ digestive tract showed a slight decrease in the number of viable bacterial cells relative to the number of microorganisms at the beginning of the experiment. As a result of successive keeping in media with pH 5.0, 3.0 and 7.0 (corresponding to the goiter, stomach and intestines), the number of viable cells of strain IV8 was 2.1x109 CFU/ml, which corresponds to 80.8% of the initial concentration of lactobacilli cells. Both strains of lactobacilli are capable of secreting extracellular hydrolytic enzymes into the medium. The highest activity of cellulase and amylase (0.52 ± 0.02 U/ml and 3.97±0.09 U/ml, respectively) was possessed by strain IV8: 1.4 and 1.3 times higher compared to the level production of these enzymes in strain IV72. Strain IV72 was characterized by a higher level of biosynthesis of protease, phytase and lipase compared to strain IV8 (respectively 0.26±0.03 U/ml, 0.33±0.02 U/ml and 4.24±0,15 U/ml), the activity of which was higher by 2.2, 1.7 and 1.6 times, respectively.

Keywords: poultry farming, lactobacilli, Lactobacillus acidophilus, strain, antagonistic potential, activity of extracellular hydrolytic enzymes, probiotic supplement, nutrient medium, cultivation, antimicrobial effect.

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Author affiliation:

Valiullin Lenar R, Ph.D. in Biology, senior scientific researcher of the Department of Ecology of the All-Russian Scientific Research Institute of Phytopathology; 5, Institute st., Bolshie Vyazemy, Odintsovo district, Moscow region, 143050; phone: 8-950-9698469; e-mail: valiullin27@mail.ru.

Mukhammadiev Rinat S., Ph.D. in Biology, scientific researcher of the Department of Ecology of the All-Russian Scientific Research Institute of Phytopathology; scientific researcher of the Department of Biotechnology of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodor-2 st., Kazan, 420075; phone: 8-987-4214127; e-mail: tanirtashir@mail.ru.

Timerbaeva Razaliya R., Ph.D. in Veterinary Medicine, docent of the Department of Epizootology and Parasitology of the Kazan State Academy of Veterinary Medicine named after N.E. Bauman; 35, Sibirskiy Trakt st., Kazan 420029; phone: 8-903-3401420; e-mail: 8-903-3401420@mail.ru.

Karimullina Ilsiyar G., Ph.D. in Biology, senior scientific researcher of the Department of Virus and Ultrastructural Research of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodor-2 st., Kazan, 420075; phone: 8-904-7699225; e-mail: 89047699225@mail.ru.

Yarullin Ainur I., Ph.D. in Biology, senior scientific researcher of the Department of Virus and Ultrastructural Research of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodor-2 st., Kazan, 420075; phone: 8-905-3174170; e-mail: abii@mail.ru.

Responsible for correspondence with the editorial board: Mukhammadiev Rishat S., Ph.D. in Biology, scientific researcher of the Department of Ecology of the All-Russian Scientific Research Institute of Phytopathology; scientific researcher of the Department of Biotechnology of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodor-2 st., Kazan,