ÓÄÊ: 57.083.33:616.98:578.826:636.592
DOI 10.33861/2071-8020-2025-5-33-35
Original Empirical Research
Kraskov D. A., Javadov E. D., Veretennikov V. V., Tarlavin N. V.
Abstract. Currently, in the Russian Federation, turkey farming is one of the most profitable sectors of the poultry industry. In addition to diseases common to all poultry (Newcastle disease, avian influenza, infectious encephalomyelitis), turkey farming faces specific diseases, one of which is hemorrhagic enteritis of turkeys. This immunosuppressive disease is increasingly being detected on turkey farms in Russia, leading to economic losses. Unfortunately, the Russian market has only one registered enzyme-linked immunosorbent assay (ELISA) kit for detecting antibodies in turkey serum. The objective of this study was to develop components for serological reactions, both for the detection of antibodies and for the detection of hemorrhagic enteritis virus antigen in pathological material. The article describes methods for obtaining positive and negative antigens and antibodies against turkey hemorrhagic enteritis virus. This will enable scientific research to advance in the development of serological tests such as agar gel immunodiffusion (AGID), latex agglutination test (LAT), immunoelectroosmophoresis (IEOP), and ELISA. Testing these components in the agar gel immunodiffusion and latex agglutination tests yielded positive results. This indicates that in the future, the obtained antigens and antibodies against turkey hemorrhagic enteritis virus can be used to create various serological kits.
Keywords: hemorrhagic enteritis of turkeys, serological reactions, antigen, antibodies, agar gel immunodiffusion, latex agglutination test.
Author affiliation:
DzhavadovEduardD.,Academicianof theRussianAcademy ofSciences, D.Sc. in Veterinary Medicine, Professor of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint Petersburg, 196084; phone: 8-921-9666774; e-mail: vnivip1@mail.ru.
Veretennikov Vladislav V., Ph.D. in Veterinary Medicine, Senior Lecturer of the Department of Epizootology named after V. P. Urban Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint Petersburg, 196084; phone: 8-981-8778769; e-mail: vlad. veretennikov.96@mail.ru.
Tarlavin Nikolay V., Ph.D. in Veterinary Medicine, Senior Lecturer of the Department of Epizootology named after V. P. Urban Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint Petersburg, 196084; phone: 8-981-8430353; e-mail: tarlav1995@bk.ru.
Responsible for correspondence with the editorial board: Kraskov Dmitry A., Assistant of the Department of Epizootology named after V.P. Urban of the Department of Epizootology named after V. P. Urban Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint Petersburg, 196084; phone: 8-921-4152266; e-mail: kraskov-00@bk.ru.
Author Contributions:
Kraskov D. A.: conceptualization, investigation, methodology, visualization.
Dzhavadov E. D.: supervision.
Veretennikov V. V.: investigation, funding acquisition.
Tarlavin N. V.: resources, validation.
Conflict of Interest Statement: the authors declare no conflict of interest.
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