Comparitive measurment of import and domestic nutrient medium growing properties for brucellosis vaccines quality control

УДК: 579.841.93
DOI 10.33861/2071-8020-2025-5-23-25

Original Empirical Research

Roenko A. D., Pimenov N. V.

Abstract. Brucellosis is a socially significant zoonotic infection affecting farm animals and transmitted to humans through products from infected animals. Brucellosis is widespread in the CIS countries. The main means of preventing brucellosis is vaccination. In the Russian Federation, vaccines represented by S-forms of brucellas are used to combat brucellosis infection. However, recently, vaccines represented only by R-forms have also become popular. The advantages of such vaccines are the ability to differentiate infected animals from vaccinated ones, and the fact that such vaccines have lower residual virulence, which allows vaccinating animals, including in farms unfavorable for brucellosis, without the risk of developing the disease. To control the quality of live attenuated vaccines, nutrient media of a certain composition is used. A certain number of doses in vials of vaccine are obtained from a number of colonies grown on plates with nutrient protection. In the Russian Federation, there is a need for import substitution of such products. However, when choosing a domestic nutrient medium for quality control, it is necessary that it ensures stable growth rates of processes, not inferior in this aspect to imported analogues. Vaccines made from the R-form of Brucella are more sensitive to cultivation parameters, including the composition of agars for quality control of brucellosis vaccines. Therefore, in our work, for a comparative assessment of the growth of the properties of nutrients among domestic and imported production, we used the Brucella abortus strain RB-51 ‘Schelkovskiy biocombinat’, represented only by stable R-forms.

Keywords: vaccines, immunoprofilactics, import substitution, brucellosis, quality control, nutrient medium, brucellagar, growing properties.

Author affiliation:

Pimenov Nikolay V., D.Sc. in Biology, Professor, Head of the Department of Immunology and Biotechnology of the Moscow State Academy of Veterinary Medicine and Biotechnology – MVA named after K.I. Skryabin; e-mail: pimenov-nikolai@yandex.ru.

Responsible for correspondence with the editorial board: Roenko Andrey D., postgraduate student of the Department of Immunology and Biotechnology of the Moscow State Academy of Veterinary Medicine and Biotechnology – MVA named after K.I. Skryabin; phone: 8-916-2315723; e-mail: rond900@gmail.com.

Authors’ Contribution: the manuscript was written with the input of all authors. All authors approved the final version of the manuscript.

Conflict of Interest Statement: the authors declare no conflict of interest.