Detection of Mycoplasma Hyopneumoniae DNA in porcine bronchioalveolar lavage and saliva

DOI 10.33861/2071-8020-2021-5-16-18

Raev S.A., Stafford V.V., Yuzhakov A.G., Komina A.K., Streltsova Y.B., Kucheruk O.D., Anoyatbekov M., Aliper T.I.

Summary. Mycoplasma hyopneumoniae – is a causative agent of the enzootic pneumonia of pigs, a major pathogen of porcine respiratory disease complex which is ubiquitous, especially in regions with intensive swine industry. Investigation was done in 3 industry-type farms, located in Uralsky and Siberian Federal districts. Biological material was taken from pigs of various age and sows. Bronchoalveolar lavage and saliva were used as samples for PCR (n=10 for each group). Blood serum samples were used for antibody detection against M. hyopneumoniae (n=10 for each group). It was found that propotion of positive samples between farms was vary widely. In the 1st farm positive samples have only been presented by saliva samples. In contrast, in the 3rd farm all positive samples (45% of the total) were bronchioalveolar lavage samples. Finally, almost equal positive rate between saliva and bronchioalveolar lavage samples has been found at the 2nd farm. Thus, variation detected in the results of comparative study of the detection on M. hyopneumoniae in saliva and bronchioalveolar lavage samples did not allow us to make a conclusion that one of these materials has a clear advantages over the second one, that would be represented in all farms studied. Moreover, antibody detection against M. hyopneumoniae data did not demonstrate any significant differences between farms investigated. Therefore, both materials should be used in screening PCR studies.

Keywords: pigs, pig complex, porcine enzootic pneumonia, Mycoplasma hyopneumoniae, prevalence, monitoring, bronchoalveolar lavage, saliva, blood serum, PCR.

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Author affiliation:

Raev Sergey A., Ph.D. in Biology, Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I.

Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Yuzhakov Anton G., Ph.D. in Biology; senior scientific researcher of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Komina Alina K., junior scientific researcher of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Streltsova Yana B., junior scientific researcher of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Kucheruk Oksana D., Ph.D. in Veterinary Medicine, leading scientific researcher of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R.

Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Anoyatbekov Muzafarbek, D.Sc. in Veterinary Medicine, Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Aliper Taras I., D.Sc. in Biology, professor, Head of the Laboratory for Epizootology, Diagnostics and Prevention of Viral Diseases of Pigs of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428.

Responsible for correspondence with the editorial board: Stafford Viktoriya V., Ph.D. in Biology, Leading Scientific Researcher of the Pathamorphology Sector of the Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after V.I. K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences; 24-1, Ryazanskiy ave., Moscow, Russia, 109428; phone: 8-926-4382045; e-mail: stafford.v.v@gmail.com.