Dynamics of antimicrobial resistance of enterococcus on a commercial dairy farm

DOI 10.33861/2071-8020-2021-4-9-12

Krivonogova A.S., Isaeva A.G., Sokolova O.V., Bezborodova N.A., Moiseeva K.V.

Summary. The studies of phenotypic sensitivity dynamics of Enterococcus faecium and Enterococcus faecalis isolates to antibiotics were conducted on a dairy farm as part of the research work on the analysis of the contamination of livestock microbiomes with antimicrobial resistance agents. Authors evaluated the susceptibility of enterococci to vancomycin, meropenem, enrofloxacin, ampicillin, inhibitor-protected amoxicillin in 2018, 2019, 2020, analyzed the changes in the resistance of isolates over a three-year observation period. It was found that in most samples, enterococci were represented by associations of E. faecium and E. faecalis. In 32-45% of cases, isolates of only one species were found in the samples, while the frequency of detection of E. faecium and E. faecalis was comparatively the same. Other types of enterococci were found in single numbers. The analysis of phenotypic antibiotic susceptibility has shown that the average level of resistance of obtained enterococcal isolates hovered around 4-15%. An increase in the number of meropenem-resistant E. faecium isolates was observed; as well as the emergence of enrofloxacin resistance in E. faecalis isolates. A high percentage (more than 10%) of penicillin-resistant isolates of E. faecium and E. faecalis were also identified; just as the rise of resistant to meropenem and vancomycin enterococci. Favorable trends were revealed in a decrease in the proportion of E. faecium isolates resistant to ampicillin, amoxicillin, and enrofloxacin. During 2018-2020 the redistribution of E. faecium and E. faecalis isolates between susceptible, moderately susceptible and resistant groups was indicated, while the main trend was found to be a decrease in the proportion of isolates with intermediate sensitivity to increased concentrations of antibiotics.

Keywords: antibiotic sensitivity, antibiotic resistance, Enterococci, Enterococcus faecium, Enterococcus faecalis, dairy farm, antimicrobials, vancomycin, meropenem, enrofloxacin, ampicillin

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Author affiliation:

Krivonogova Anna S., D.Sc. in Biology, docent, Leading Scientific Researcher of the laboratory of biological technology of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112-a, Belinskogo st., Ekaterinburg, 620145; phone: 8-343-2572044; e-mail: tel-89826512934@yandex.ru.

Sokolova Olga V., Ph.D. in Biology Sciences, Senior Scientific Researcher of the laboratory of genetic research and animal selection of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112-a, Belinskogo st., Ekaterinburg, 620145; phone: 8-343-2572044; e-mail: nauka_sokolova@mail.ru

Bezborodova Natalia A., Ph.D. in Veterinary Medicine, Senior Scientific Researcher of the laboratory of genetic research and animal selection of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112-a, Belinskogo st., Ekaterinburg, 620145; phone: 8-343-2572044; e-mail: n-bezborodova@mail.ru.

Moiseeva Kseniya V., Junior Scientific Researcher of the laboratory of biological technology of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112-a, Belinskogo st., Ekaterinburg, 620145; phone: 8-343-2572044; e-mail: moiseeva456@yandex.ru.

Responsible for correspondence with the editorial board: Isaeva Albina G., D.Sc. in Biology, Leading Scientific Researcher of the laboratory of biological technology of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112-a, Belinskogo st., Ekaterinburg, 620145; phone: 8-343-2572044; e-mail: isaeva.05@bk.ru.