Intestinal microbiota state in healthy cows and at necrobacteriosis

Terekhov V.I., Malysheva T.V., Kramar P.V., Skorikov A.V.

Summary

Necrobacillosis is factorial infectious disease, occurrence of which depends on the conditions and feeding of animals. The aim of research was to examine the qualitative and quantitative composition of the distal gut microflora of cows and its enzymatic activity in different physiological periods and at necrobacteriosis. At necrobacteriosis intestinal microbiota state is in marked imbalance, which is evident not only in the ratio imbalance of symbiontic, saprophytic and opportunistic pathogens, but also in a significant decrease of enzymatic activity of the intestinal microflora. At necrobacteriosis number of lactobacilli and bifidobacteria were even lower, ranging 3,7 and 4,25 lg CFU/g, when the number of micromycetes, Clostridium, Bacteroides, bacilli, enterococci and Escherichia increased by 1-2 times. So, for elimination or prevention of necrobacteriosis intestinal microbial structure must be normalized.

Key words: cow, distal intestine, intestinal microbiocenosis, dysbiosis, intestinal microflora, symbionts, necrobacteriosis.

References

1. Gimranov V.V., Uteev R.A., Gilyazov A.F. Etiologiya, kharakter rasprostranennosti i osobennosti patologii v oblasti paltsev u korov golshtinofrizskoy porody [Etiology, character of prevalence and disorder peculiarities of Holstein-Friesian cows’ fingers]. - Agrarny vestnik Urala. – Yekaterinburg, 2010 (3). – pp.77-79.
2. Kablucheeva T.I. Ispol’zovanie probiotikov v ptitsevodstve [Probiotic use in poultry breeding]. – Krasnodar, 2004. –107 p.
3. Krapivina E.V., Ivanov D.V., Ignatenko M.V. Monitoringovoe issledovanie zashchitnykh funktsiy i mikrobiotsenoza tolstogo kishechnika u laktiruyushchikh korov [Monitoring study of protective functions and microbiocenosis of the large intestine in lactating cows]. – 2010: pp. 5-10.
4. Kryazhevskikh L., Laptev G. Metod T-RFLP – osnova profilaktiki nekrobakterioza skota [T-RFLP – basic method of prevention of cattle necrobacteriosis]. – Zhivotnovodstvo Rossii. – Moscow, 2009 (10). – pp.38-40.
5. Mishchenko V.A. Analiz prichin zabolevaniy vysokoproduktivnykh korov [Analysis of causes of diseases of high yielding cows]. – Vestnik OrelGAU. – Orel, 2008 (2). – pp.21-24.
6. Shkuratova I.A., Vereshchak N.A., Belousov A.I. Rasprostranenie i osobennosti proyavleniya narusheniy obmena veshchestv u vysokoproduktivnykh korov Sverdlovskoy oblasti [Distribution and features of display of metabolic disorders in high yielding cows of Sverdlovsk area]. – Yekaterinburg, 2010. – pp.472-474.
7. Collinder Eje Intestinal functions in animals/ Stockholm, Sweden, 2001. – 67p.
8. Leroy, R.V. Normal Intestinal Flora of Cattle Fed High-Roughage Rations / R.V. Leroy, R.Kay // Journal of Bacteriology. 1965. –Vol.89. – 5. –P. 1244–1249.

Author affiliation

Kramar Pavel V., applicant of the department of microbiology, epizootiology and virology of the Kuban State Agrarian University; 13, Kalinina st., Krasnodar, 350044; phone: 8(909)46656609.

Malysheva Tatiana V., Ph.D. in Medicine, docent of the department of microbiology of the Kuban State Medical University; 4, Sedina st., Krasnodar, 350063; phone: 8(918)2422225.

Skorikov Aleksander V., Ph.D., Deputy Head of the State veterinary department of Krasnodar region; 36, Rashplevskaya st., Krasnodar, 350000; phone: 8(918)3145411.

Responsible for correspondence with the editorial board: Terekhov Vladimir I., D.Sc. in Biology, professor of the department of microbiology, epizootiology and virology of the Kuban State Agrarian University; 13, Kalinina st., Krasnodar, 350044; phone: 8(988)4742115; e-mail: vterekhov@list.ru.